Cord blood group 2 innate lymphoid cells are associated with lung function at 6 weeks of age

Clinical & Translational Immunology

Peter D Sly¹  Paul D Robinson²  Vanessa E Murphy³  Patricia de Gouveia Belinelo³  Malcolm R Starkey³  Joerg Mattes³  Adam M Collison³  Gabriela Martins Costa Gomes³  Philip M Hansbro⁴  Peter G Gibson⁴  Wilfried Karmaus⁵ 

[1] Child Health Research Centre University of Queensland Brisbane QLD Australia;Department of Respiratory Medicine The Children's Hospital at Westmead Sydney NSW Australia;Priority Research Centre GrowUpWell® – Hunter Medical Research Institute The University of Newcastle Newcastle NSW Australia;Priority Research Centre for Healthy Lungs ‐ Hunter Medical Research Institute University of Newcastle Newcastle NSW Australia;School of Public Health University of Memphis Memphis TN USA;

关键词: asthma;    cord blood;    CRTh2 ILC2;    multiple breath washout;    tidal breathing;   

DOI  :  10.1002/cti2.1296

来源: DOAJ

【 摘 要 】

Abstract Objective Offspring born to mothers with asthma in pregnancy are known to have lower lung function which tracks with age. Human group 2 innate lymphoid cells (ILC2) accumulate in foetal lungs, at 10‐fold higher levels compared to adult lungs. However, there are no data on foetal ILC2 numbers and the association with respiratory health outcomes such as lung function in early life. We aimed to investigate cord blood immune cell populations from babies born to mothers with asthma in pregnancy. Methods Cord blood from babies born to asthmatic mothers was collected, and cells were stained in whole cord blood. Analyses were done using traditional gating approaches and computational methodologies (t‐distributed stochastic neighbour embedding and PhenoGraph algorithms). At 6 weeks of age, the time to peak tidal expiratory flow as a percentage of total expiratory flow time (tPTEF/tE%) was determined as well as Lung Clearance Index (LCI), during quiet natural sleep. Results Of 110 eligible infants (March 2017 to November 2019), 91 were successfully immunophenotyped (82.7%). Lung function was attempted in 61 infants (67.0%), and 43 of those infants (70.5% of attempted) had technically acceptable tPTEF/tE% measurements. Thirty‐four infants (55.7% of attempted) had acceptable LCI measurements. Foetal ILC2 numbers with increased expression of chemoattractant receptor‐homologous molecule (CRTh2), characterised by two distinct analysis methodologies, were associated with poorer infant lung function at 6 weeks of age.” Conclusion Foetal immune responses may be a surrogate variable for or directly influence lung function outcomes in early life.

【 授权许可】

Unknown