Tea extract increases cell fusion via regulation of cell surface DC-STAMP

Biochemistry and Biophysics Reports

Tomoko Matsuda¹  Masahiro Nishio²  Hayato Umekawa³  Kenji Kuriya³ 

[1] Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577, Kurimamachiya, Tsu, Mie, 514-8507, Japan;Corresponding author. Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Mie, 514-8507, Japan.;Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577, Kurimamachiya, Tsu, Mie, 514-8507, Japan;

关键词: Epigallocatechin gallate;    Catechin;    DC-STAMP;    Tea extract;    RAW264.7;   

DOI  :  

来源: DOAJ

【 摘 要 】

Mononuclear osteoclast precursor cells fuse with each other to become mature multinucleated osteoclasts, which is regulated by dendritic cell-specific transmembrane protein (DC-STAMP). We evaluated the effects of tea extract and catechins on cell-cell fusion and DC-STAMP expression to elucidate their relationship with osteoclast development. When tea extract or epigallocatechin gallate (EGCg) was applied to RAW264.7 cells, multinucleated cells were increased significantly, while tartrate-resistant acid phosphatase (TRAP) activity was hardly upregulated. Flow cytometric analysis revealed that EGCg suppressed DC-STAMP expression on the cell surface, which is similar to osteoclast development. These observations suggest that TRAP activity is not activated even when suppression of both surface DC-STAMP expression and multinucleation occurs, which might be mediated by another pathway.

【 授权许可】

Unknown