Field-Evolved ΔG210-ppo2 from Palmer Amaranth Confers Pre-emergence Tolerance to PPO-Inhibitors in Rice and Arabidopsis

Genes

Steven J. Bowe¹  Aimone Porri²  Jens Lerchl²  Ana Claudia Langaro³  Gulab Rangani⁴  Nilda Roma-Burgos⁴  Vibha Srivastava⁴  Pamela Carvalho-Moore⁴ 

[1] BASF Agricultural Solutions, Research Triangle Park, NC 27713, USA;BASF SE, RP 67056 Limburgerhof, Germany;Corteva Agriscience, Nova Mutum 78450, MT, Brazil;Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72704, USA;

关键词: transgenic rice;    Amaranthus palmeri;    fomesafen;    protoporphyrinogen oxidase;    herbicide resistance;    root growth inhibition;   

DOI  :  10.3390/genes13061044

来源: DOAJ

【 摘 要 】

Resistance to protoporphyrinogen IX oxidase (PPO)-inhibitors in Amaranthus palmeri and Amaranthus tuberculatus is mainly contributed by mutations in the PPO enzyme, which renders herbicide molecules ineffective. The deletion of glycine210 (ΔG210) is the most predominant PPO mutation. ΔG210-ppo2 is overexpressed in rice (Oryza sativa c. ‘Nipponbare’) and Arabidopsis thaliana (Col-0). A foliar assay was conducted on transgenic T₁ rice plants with 2× dose of fomesafen (780 g ha⁻¹), showing less injury than the non-transgenic (WT) plants. A soil-based assay conducted with T₂ rice seeds confirmed tolerance to fomesafen applied pre-emergence. In agar medium, root growth of WT rice seedlings was inhibited >90% at 5 µM fomesafen, while root growth of T2 seedlings was inhibited by 50% at 45 µM fomesafen. The presence and expression of the transgene were confirmed in the T₂ rice survivors of soil-applied fomesafen. A soil-based assay was also conducted with transgenic A. thaliana expressing ΔG210-ppo2 which confirmed tolerance to the pre-emergence application of fomesafen and saflufenacil. The expression of A. palmeri ΔG210-ppo2 successfully conferred tolerance to soil-applied fomesafen in rice and Arabidopsis. This mutant also confers cross-tolerance to saflufenacil in Arabidopsis. This trait could be introduced into high-value crops that lack chemical options for weed management.

【 授权许可】

Unknown