Epitope‐based peptide vaccine design and target site depiction against Middle East Respiratory Syndrome Coronavirus: an immune-informatics study

Journal of Translational Medicine

Muhammad Tahir ul Qamar¹  Amna Bari¹  Usman Ali Ashfaq²  Saman Saleem²  Farooq Anwar³  Safar Alqahtani⁴ 

[1] College of Informatics, Huazhong Agricultural University;Department of Bioinformatics and Biotechnology, Government College University Faisalabad;Department of Chemistry, University of Sargodha;Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdul Aziz University;

关键词: MERS-COV;    Spike protein;    T-and B-cell epitopes;    Computational approaches;    Vaccine design;   

DOI  :  10.1186/s12967-019-2116-8

来源: DOAJ

【 摘 要 】

Abstract Background Middle East Respiratory Syndrome Coronavirus (MERS-COV) is the main cause of lung and kidney infections in developing countries such as Saudi Arabia and South Korea. This infectious single-stranded, positive (+) sense RNA virus enters the host by binding to dipeptidyl-peptide receptors. Since no vaccine is yet available for MERS-COV, rapid case identification, isolation, and infection prevention strategies must be used to combat the spreading of MERS-COV infection. Additionally, there is a desperate need for vaccines and antiviral strategies. Methods The present study used immuno-informatics and computational approaches to identify conserved B- and T cell epitopes for the MERS-COV spike (S) protein that may perform a significant role in eliciting the resistance response to MERS-COV infection. Results Many conserved cytotoxic T-lymphocyte epitopes and discontinuous and linear B-cell epitopes were predicted for the MERS-COV S protein, and their antigenicity and interactions with the human leukocyte antigen (HLA) B7 allele were estimated. Among B-cell epitopes, QLQMGFGITVQYGT displayed the highest antigenicity-score, and was immensely immunogenic. Among T-cell epitopes, MHC class-I peptide YKLQPLTFL and MHC class-II peptide YCILEPRSG were identified as highly antigenic. Furthermore, docking analyses revealed that the predicted peptides engaged in strong bonding with the HLA-B7 allele. Conclusion The present study identified several MERS-COV S protein epitopes that are conserved among various isolates from different countries. The putative antigenic epitopes may prove effective as novel vaccines for eradication and combating of MERS-COV infection.

【 授权许可】

Unknown