Vaccine design of coronavirus spike (S) glycoprotein in chicken: immunoinformatics and computational approaches

Translational Medicine Communications

Sumaia A. Ali¹  Eman A. Awadelkareem² 

[1] Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology;Faculty of Veterinary Medicine, University of Khartoum;

关键词: IBV;    Spike protein;    B-and T-cell epitopes;    Computational approaches;    Vaccine design;   

DOI  :  10.1186/s41231-020-00063-0

来源: DOAJ

【 摘 要 】

Abstract Background Infectious bronchitis (IB) is a highly contagious respiratory disease in chickens and produces economic loss within the poultry industry. This disease is caused by a single stranded RNA virus belonging to Cronaviridae family. This study aimed to design a potential multi-epitopes vaccine against infectious bronchitis virus spike protein (S). Protein characterization was also performed for IBV spike protein. Methods The present study used various tools in Immune Epitope Database (IEDB) to predict conserved B and T cell epitopes against IBV spike (S) protein that may perform a significant role in provoking the resistance response to IBV infection. Results In B cell prediction methods, three epitopes ( 1139 KKSSYY 1144 , 1140 KSSYYT 1145 , 1141 SSYYT 1145 ) were selected as surface, linear and antigenic epitopes. Many MHCI and MHCII epitopes were predicted for IBV S protein. Among them 982YYITARDMY990 and 983 YITARDMYM 991 epitopes displayed high antigenicity, no allergenicity and no toxicity as well as great linkage with MHCI and MHCII alleles. Moreover, docking analysis of MHCI epitopes produced strong binding affinity with BF2 alleles. Conclusion Five conserved epitopes were expected from spike glycoprotein of IBV as the best B and T cell epitopes due to high antigenicity, no allergenicity and no toxicity. In addition, MHC epitopes showed great linkage with MHC alleles as well as strong interaction with BF2 alleles. These epitopes should be designed and incorporated and then tested as multi-epitope vaccine against IBV.

【 授权许可】

Unknown