Dominant integration locus drives continuous diversification of plant immune receptors with exogenous domain fusions

Genome Biology

Christian Schudoma¹  Wilfried Haerty¹  Ksenia V. Krasileva¹  Erin Baggs¹  Paul C. Bailey¹  William Jackson²  Matthew Moscou²  Gulay Dagdas² 

[1] Earlham Institute, Norwich Research Park;The Sainsbury Laboratory, Norwich Research Park;

关键词: Plant immunity;    Disease resistance genes;    NLRs;    Gene fusions;   

DOI  :  10.1186/s13059-018-1392-6

来源: DOAJ

【 摘 要 】

Abstract Background The plant immune system is innate and encoded in the germline. Using it efficiently, plants are capable of recognizing a diverse range of rapidly evolving pathogens. A recently described phenomenon shows that plant immune receptors are able to recognize pathogen effectors through the acquisition of exogenous protein domains from other plant genes. Results We show that plant immune receptors with integrated domains are distributed unevenly across their phylogeny in grasses. Using phylogenetic analysis, we uncover a major integration clade, whose members underwent repeated independent integration events producing diverse fusions. This clade is ancestral in grasses with members often found on syntenic chromosomes. Analyses of these fusion events reveals that homologous receptors can be fused to diverse domains. Furthermore, we discover a 43 amino acid long motif associated with this dominant integration clade which is located immediately upstream of the fusion site. Sequence analysis reveals that DNA transposition and/or ectopic recombination are the most likely mechanisms of formation for nucleotide binding leucine rich repeat proteins with integrated domains. Conclusions The identification of this subclass of plant immune receptors that is naturally adapted to new domain integration will inform biotechnological approaches for generating synthetic receptors with novel pathogen “baits.”

【 授权许可】

Unknown