A bZIP transcription factor, PqbZIP1, is involved in the plant defense response of American ginseng

PeerJ

article

Shanshan Yang¹  Xiaoxiao Zhang²  Ximei Zhang¹  Yanmeng Bi¹  Weiwei Gao¹ 

[1] Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;College of Agriculture, Guangxi University;Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences;School of Environmental and Municipal Engineering, Tianjin Chengjian University

关键词: American ginseng;    Panax quinquefolius L.;    PqbZIP1;    Root rot disease;    Fusarium solani;    Immune response;    Disease resistance genes;   

DOI  :  10.7717/peerj.12939

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

American ginseng (Panax quinquefolius L.) is a perennial medicinal plant that has a long usage history in China. However, root rot, which is mainly caused by Fusarium solani can severely reduce the yield and quality of American ginseng, but no disease-resistant variety of American ginseng exists, and the resistance against this disease is not yet well understood. Thus, it is very urgent to analyze the interaction mechanism regulating the interactions between American ginseng and F. solani to mine disease resistance genes. Using transcriptome data and quantitative polymerase chain reaction (qPCR), we screened the transcription factor PqbZIP1 in response to induction by chitin. Yeast self-activation and subcellular localization experiments proved that PqbZIP1 showed transcriptional activity and was localized in the plant nucleus. In addition, qPCR showed that the highest relative expression level was in the roots, wherein chitin and F. solani inhibited and activated the expression of PqbZIP1, respectively, in American ginseng. Additionally, PqbZIP1 significantly inhibited the growth of the Pseudomonas syringae pv. tomato D36E strain in Nicotiana benthamiana, where expressing PqbZIP1 in N. benthamiana increased the jasmonic acid, salicylic acid, and abscisic acid content. Furthermore, PqbZIP1 expression was continually increased upon inoculation with F. solani. Hence, this study revealed that the PqbZIP1 transcription factor might mediate multiple hormonal signaling pathway to modulate root rot disease resistance in American ginseng, and provided important information to breed disease-resistant American ginseng.

【 授权许可】

CC BY