MiRNA Profiles of Extracellular Vesicles Secreted by Mesenchymal Stromal Cells—Can They Predict Potential Off-Target Effects?

Biomolecules

Martina Seifert¹  ChristienM. Beez¹  Katrin Krüger²  Sebastian Neuber³  TimoZ. Nazari-Shafti³  Volkmar Falk³  Vasileios Exarchos³  Heike Meyborg³  MaximilianY. Emmert³  AnaG. Duran³  Johanna Buschmann⁴  Petra Wolint⁴  Roland Böni⁵ 

[1] Berlin Institute of Health Center for Regenerative Therapies, Charité—Universitätsmedizin Berlin, 13353 Berlin, Germany;Clinic for Cardiovascular Surgery, Charité—Universitätsmedizin Berlin, 13353 Berlin, Germany;Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, 13353 Berlin, Germany;Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 Zurich, Switzerland;White House Center for Liposuction, 8044 Zurich, Switzerland;

关键词: mesenchymal stromal cells;    extracellular vesicles;    microRNA;    oncomiR;    tumor suppressor;    cardioprotection;   

DOI  :  10.3390/biom10091353

来源: DOAJ

【 摘 要 】

The cardioprotective properties of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) are currently being investigated in preclinical studies. Although microRNAs (miRNAs) encapsulated in EVs have been identified as one component responsible for the cardioprotective effect of MSCs, their potential off-target effects have not been sufficiently characterized. In the present study, we aimed to investigate the miRNA profile of EVs isolated from MSCs that were derived from cord blood (CB) and adipose tissue (AT). The identified miRNAs were then compared to known targets from the literature to discover possible adverse effects prior to clinical use. Our data show that while many cardioprotective miRNAs such as miR-22-3p, miR-26a-5p, miR-29c-3p, and miR-125b-5p were present in CB- and AT-MSC-derived EVs, a large number of known oncogenic and tumor suppressor miRNAs such as miR-16-5p, miR-23a-3p, and miR-191-5p were also detected. These findings highlight the importance of quality assessment for therapeutically applied EV preparations.

【 授权许可】

Unknown