Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing

Journal of Functional Foods

Ana María González-Paramás¹  Massimiliano Gasparrini²  Francesca Giampieri³  Celestino Santos-Buelga⁴  Tamara Yuliet Forbes-Hernández⁵  Maurizio Battino⁶  Pablo Beltrán-Ayala⁷  Mario Cordero⁷  José Luis Quiles⁷  José Miguel Alvarez-Suarez⁷  Sadia Afrin⁷  Alfonso Varela-Lopez⁸  Luca Mazzoni⁹ 

[1] Centre for Nutrition &Corresponding authors. DISCO, Facoltà di Medicina, Università Politecnica delle Marche, Via Ranieri 65, 60131 Ancona, Italy. Tel.: [phone];Escuela de Medicina Veterinaria y Zootecnia, Facultad de Ciencias de la Salud, Universidad de Las Américas (UDLA), Quito, Ecuador;Health, Universidad Europea del Atlantico, Santander, Spain;fax: [phone].;Colegio de Administración y Economía (CADE), Universidad San Francisco de Quito (USFQ), Quito, Ecuador;Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche (DISCO), Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy;Dipartimento di Scienze Agrarie, Alimentari e Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy;Research Laboratory, Dental School, University of Sevilla, Sevilla, Spain;

关键词: Manuka honey;    ROS;    AMPK;    Nrf2;    Wound healing;   

DOI  :  

来源: DOAJ

【 摘 要 】

Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response.

【 授权许可】

Unknown