期刊论文详细信息
Journal of Fungi
Effects of Oleic Acid Addition Methods on the Metabolic Flux Distribution of Ganoderic Acids R, S and T’s Biosynthesis
Li-Ping Liu1  Jie Feng1  Shuai Zhou1  Yi Tan1  Chuan-Hong Tang1  Jing-Song Zhang1  Qing-Jiu Tang1  Meng-Qiu Yan1  Yan-Fang Liu1  Xiao-Wei Su1 
[1] Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, China;
关键词: Ganoderma lucidum;    submerged fermentation-static culture;    oleic acid;    metabolic flux analysis;    ganoderic acid;   
DOI  :  10.3390/jof8060615
来源: DOAJ
【 摘 要 】

The effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids R, S and T’s biosynthesis from Ganoderma lucidum were investigated. The results showed that adding filter-sterilized oleic acid in the process of submerged fermentation and static culture is of benefit to the synthesis of ganoderic acids R, S and T. The metabolic fluxes were increased by 97.48%, 78.42% and 43.39%, respectively. The content of ganoderic acids R, S and T were 3.11 times, 5.19 times and 1.44 times higher, respectively, than they were in the control group, which was without additional oleic acid. Ganoderic acids R, S and T’s synthesis pathways (GAP), tricarboxylic acid cycles (TCA), pentose phosphate pathways (PP) and glycolysis pathways (EMP) were all enhanced in the process. Therefore, additional oleic acid can strengthen the overall metabolic flux distribution of G. lucidum in a submerged fermentation-static culture and it can reduce the accumulation of the by-product mycosterol. This study has laid an important foundation for improving the production of triterpenes in the submerged fermentation of G. lucidum.

【 授权许可】

Unknown   

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