Water-Extracted Ganoderma lucidum Induces Apoptosis and S-Phase Arrest via Cyclin-CDK2 Pathway in Glioblastoma Cells

Molecules

Yi-Hsien Hsieh¹  Yi-Chung Chien²  Han-Chung Lee³  An-Yi Cheng⁴  Yung-Luen Yu⁴ 

[1] Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan;Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan;School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan;The Ph.D. Program of Biotechnology and Biomedical Industry, China Medical University, Taichung 40402, Taiwan;

关键词: glioblastoma;    Ganoderma lucidum;    apoptosis;    S phase arrest;    CDK2;   

DOI  :  10.3390/molecules25163585

来源: DOAJ

【 摘 要 】

Glioblastoma is one of the most common and most aggressive brain cancers. The current treatment is mainly surgery, chemotherapy, and radiation therapy, but the results are not satisfactory. Ganoderma lucidum (G. lucidum), also called “Lingzhi”, is a medicinal mushroom that has been used as a therapeutic agent for the treatment of numerous diseases, including cancer. However, whether it is effective for treating cancer is still unclear. In the present study, the anti-tumor effect of a water extract of G. lucidum was investigated using brain tumor cells. We used an analysis of cell viability, flow cytometry, the IncuCyte live-cell analysis system, and Western blotting to study its effects. The water extract from G. lucidum inhibited cell proliferation in a dose- and time-dependent manner, and it induced mitochondria-mediated apoptosis and cell cycle arrest at S phase via the cyclin-CDK2 pathway in human brain tumor cells. In addition, the G. lucidum extract significantly inhibited cell migration and mesenchymal marker expression based on the IncuCyte live-cell assay and qRT-PCR analysis. In summary, these anti-tumor effects in brain tumor cells suggest that G. lucidum may be useful for treating brain tumors.

【 授权许可】

Unknown