ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells

Cells

Markus Langhans¹  Franz Rödel²  Stephanie Hehlgans²  Robert Lehn³  Ayele T. Tsedeke⁴  Felicitas Rapp⁴  Jessica Molendowska⁴  Denise Eckert⁴  Claudia Fournier⁴ 

[1] Department of Membrane Dynamics, Technical University Darmstadt, Alarich-Weiss-Straße 8, 64287 Darmstadt, Germany;Department of Radiotherapy and Oncology, University Hospital, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany;Ernst-Berl Institut für Technische und Makromolekulare Chemie, Technical University Darmstadt, Alarich-Weiss-Straße 8, 64287 Darmstadt, Germany;GSI Helmholtz Center for Heavy Ion Research, Department of Biophysics, Planckstraße 1, 64291 Darmstadt, Germany;

关键词: adhesion;    endothelial cells;    inflammation;    leukocytes;    low-dose irradiation;    shear stress;   

DOI  :  10.3390/cells11010072

来源: DOAJ

【 摘 要 】

Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.

【 授权许可】

Unknown