Quantitative Proteomics Analysis of Lettuce (Lactuca sativa L.) Reveals Molecular Basis-Associated Auxin and Photosynthesis with Bolting Induced by High Temperature

International Journal of Molecular Sciences

Jing-Hong Hao¹  Xiao-Xiao Qin¹  Lu Wang¹  Shuang Xiao¹  Pan-Pan Li¹  Chao-Jie Liu¹  Zheng-Yang Qi¹  Li-Li Zhang¹  Yan-Chuan Sun¹  Ying-Yan Han¹  Shuang-Xi Fan¹  Jian-Ke Li² 

[1] Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China;Institute of Apicultural Research, Chinese Academy of Agricultural Science, No. 1 Beigou Xiangshan, Beijing 100093, China;

关键词: lettuce;    bolting;    proteome;    high temperature;    iTRAQ;   

DOI  :  10.3390/ijms19102967

来源: DOAJ

【 摘 要 】

Bolting is a key process in the growth and development of lettuce (Lactuca sativa L.). A high temperature can induce early bolting, which decreases both the quality and production of lettuce. However, knowledge of underlying lettuce bolting is still lacking. To better understand the molecular basis of bolting, a comparative proteomics analysis was conducted on lettuce stems, during the bolting period induced by a high temperature (33 °C) and a control temperature (20 °C) using iTRAQ-based proteomics, phenotypic measures, and biological verifications using qRT-PCR and Western blot. The high temperature induced lettuce bolting, while the control temperature did not. Of the 5454 identified proteins, 619 proteins presented differential abundance induced by high-temperature relative to the control group, of which 345 had an increased abundance and 274 had a decreased abundance. Proteins with an abundance level change were mainly enriched in pathways associated with photosynthesis and tryptophan metabolism involved in auxin (IAA) biosynthesis. Moreover, among the proteins with differential abundance, proteins associated with photosynthesis and tryptophan metabolism were increased. These findings indicate that a high temperature enhances the function of photosynthesis and IAA biosynthesis to promote the process of bolting, which is in line with the physiology and transcription level of IAA metabolism. Our data provide a first comprehensive dataset for gaining novel understanding of the molecular basis underlying lettuce bolting induced by high temperature. It is potentially important for further functional analysis and genetic manipulation for molecular breeding to breed new cultivars of lettuce to restrain early bolting, which is vital for improving vegetable quality.

【 授权许可】

Unknown