cfNOMe — A single assay for comprehensive epigenetic analyses of cell-free DNA

Genome Medicine

Malte P. Bartram¹  Florian Erger²  Mohammad R. Toliat²  Peter Nürnberg²  Christian Becker²  Janine Altmüller²  Domenica Borchert²  Deborah Nörling²  Michael S. Wiesener³  Esther Leenen⁴  Sandra Habbig⁵  Bodo B. Beck⁶  Andrea Wenzel⁶ 

[1] Center for Molecular Medicine Cologne (CMMC), University of Cologne;Cologne Center for Genomics, University of Cologne;Department of Nephrology and Hypertension, Friedrich-Alexander University Erlangen-Nürnberg;Department of Nephrology, Transplantation and Medical Intensive Care, University Witten/Herdecke, Medical Center Cologne-Merheim;Department of Pediatrics, Faculty of Medicine and University Hospital Cologne;Institute of Human Genetics, Faculty of Medicine and University Hospital Cologne;

关键词: cfNOMe;    Cell-free DNA;    Methylome;    Nucleosome footprints;    Analysis software;   

DOI  :  10.1186/s13073-020-00750-5

来源: DOAJ

【 摘 要 】

Abstract Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We present cfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking, cfNOMe delivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling. cfNOMe has the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.

【 授权许可】

Unknown