Diphlorethohydroxycarmalol Isolated from Ishige okamurae Represses High Glucose-Induced Angiogenesis In Vitro and In Vivo

Marine Drugs

BoMi Ryu¹  K.H. N. Fernando¹  You-Jin Jeon¹  Yunfei Jiang¹  Hye-Won Yang¹ 

[1]Department of Marine Life Science, Jeju National University, Jeju 63243, Korea

关键词: diphlorethohydroxycarmalol;    Ishige okamurae;    angiogenesis;    diabetes;    EA.hy926;    zebrafish;    VEGFR-2;   

DOI  :  10.3390/md16100375

来源: DOAJ

【 摘 要 】

Diabetes mellitus causes abnormalities of angiogenesis leading to vascular dysfunction and serious pathologies. Diphlorethohydroxycarmalol (DPHC), which is isolated from Ishige okamurae, is well known for its bioactivities, including antihyperglycemic and protective functions against diabetes-related pathologies. In the present study, the inhibitory effect of DPHC on high glucose-induced angiogenesis was investigated on the human vascular endothelial cell line EA.hy926. DPHC inhibited the cell proliferation, cell migration, and tube formation in cells exposed to 30 mM of glucose to induce angiogenesis. Furthermore, the effect of DPHC against high glucose-induced angiogenesis was evaluated in zebrafish embryos. The treatment of embryos with DPHC suppressed high glucose-induced dilation in the retinal vessel diameter and vessel formation. Moreover, DPHC could inhibit high glucose-induced vascular endothelial growth factor receptor 2 (VEGFR-2) expression and its downstream signaling cascade. Overall, these findings suggest that DPHC is actively involved in the suppression of high glucose-induced angiogenesis. Hence, DPHC is a potential agent for the development of therapeutics against angiogenesis induced by diabetes.

【 授权许可】

Unknown