The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species

Viruses

DavidJ. Holland¹  RobinM. MacDiarmid²  MahjoubA. Ejmal²  MichaelN. Pearson² 

[1]Infectious Diseases Unit, Division of Medicine, Middlemore Hospital, Auckland 1640, New Zealand

[2]School of Biological Sciences, the University of Auckland, Auckland 1142, New Zealand

关键词: chrysovirus;    mycovirus;    Aspergillus;    A. fumigatus;    A. nidulans;    A. niger;    A. thermomutatus;    biocontrol;   

DOI  :  10.3390/v10100539

来源: DOAJ

【 摘 要 】

This study determined the effects of Aspergillus thermomutatus chrysovirus 1 (AthCV1), isolated from Aspergillus thermomutatus, on A. fumigatus, A. nidulans and A. niger. Protoplasts of virus-free isolates of A. fumigatus, A. nidulans and A. niger were transfected with purified AthCV1 particles and the phenotype, growth and sporulation of the isogenic AthCV1-free and AthCV1-infected lines assessed at 20 °C and 37 °C and gene expression data collected at 37 °C. AthCV1-free and AthCV1-infected A. fumigatus produced only conidia at both temperatures but more than ten-fold reduced compared to the AthCV1-infected line. Conidiation was also significantly reduced in infected lines of A. nidulans and A. niger at 37 °C. AthCV1-infected lines of A. thermomutatus and A. nidulans produced large numbers of ascospores at both temperatures, whereas the AthCV1-free line of the former did not produce ascospores. AthCV1-infected lines of all species developed sectoring phenotypes with sclerotia produced in aconidial sectors of A. niger at 37 °C. AthCV1 was detected in 18% of sclerotia produced by AthCV1-infected A. niger and 31% of ascospores from AthCV1-infected A. nidulans. Transcriptome analysis of the naturally AthCV1-infected A. thermomutatus and the three AthCV1-transfected Aspergillus species showed altered gene expression as a result of AthCV1-infection. The results demonstrate that AthCV1 can infect a range of Aspergillus species resulting in reduced sporulation, a potentially useful attribute for a biological control agent.

【 授权许可】

Unknown