Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier

Tropical Medicine and Infectious Disease

article

Marko Jankovic¹  Valentina Cirkovic²  Gorana Stamenkovic³  Ana Loncar⁴  Marija Todorovic¹  Maja Stanojevic¹  Marina Siljic¹ 

[1] Faculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade;Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade;Department for Genetic Research, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade;Institute for Biocides and Medical Ecology

关键词: Culex pipiens;    mosquito;    arthropod-borne;    RNA virus;    mycovirus;    chrysovirus;    sequencing;    Serbia;   

DOI  :  10.3390/tropicalmed8040225

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Here, we report on a serendipitous finding of a chryso-like virus associated with Culex pipiens mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV NS5 gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution.

【 授权许可】

CC BY