Establishment of Epidemiological Resistance Cut-Off Values of Aquatic Aeromonas to Eight Antimicrobial Agents

Microorganisms

Lijian Ouyang¹  Aihua Li²  Shuyi Wang²  Jingwen Hao²  Jicheng Yang²  Yaoyao Lin²  Zhenbing Wu²  Qianqian Zhang² 

[1] Ecological Engineering College, Guizhou University of Engineering Science, Bijie 551700, China;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;

关键词: Aeromonas;    epidemiological cut-off values;    resistance rate;    MIC₅₀;    MIC₉₀;   

DOI  :  10.3390/microorganisms10040776

来源: DOAJ

【 摘 要 】

The abuse of antibiotics in aquaculture has led to the increasing rate of antibiotic resistance of aquatic bacteria including Aeromonas, which is an increasing threat to environmental and human health. To date, no epidemiological cut-off values (CO_WT) for Aeromonas spp. have been established by the Clinical and Laboratory Standards Institute nor the European Commission on Antimicrobial Susceptibility Testing. In this study, commercially prepared minimum inhibitory concentration (MIC) test 96-well plates (dry-form plates) were used to determine the MIC of eight antimicrobial agents against 556 Aeromonas strains. The obtained MIC distributions were simulated and analyzed by NRI and ECOFFinder to obtain tentative CO_WT values for Aeromonas spp. The CO_WT values of eight kinds of representative antimicrobial agents including trimethoprim–sulfamethoxazole, erythromycin, doxycycline, neomycin, colistin, florfenicol, enrofloxacin, and ceftazidime for Aeromonas spp. were established and were 0.25, 64/32, 4/2, 8, 4, 1, 0.062/0.125, and 0.5 μg/mL, respectively. Results showed that Aeromonas spp. had a very high proportion of non-wild-type strains to enrofloxacin, florfenicol, and doxycycline, which are the most widely used antimicrobials in aquaculture. The CO_WT values for Aeromonas spp. obtained in this study can contribute to the final establishment of CO_WT for Aeromonas spp. internationally.

【 授权许可】

Unknown