Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters

Sensors

Teppo Salminen¹  Sherif Bayoumy¹  Kim Pettersson¹  Etvi Juntunen¹  Karoliina Vuorenpää¹  SheikhM. Talha¹  Iida Martiskainen¹  Tytti Vuorinen²  Navin Khanna³  Gaurav Batra⁴ 

[1] Department of Biotechnology, University of Turku, 20520 Turku, Finland;Department of Virology and Clinical Microbiology, University of Turku, 20520 Turku, Finland;International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India;Translational Health Science and Technology Institute, Faridabad 121001, India;

关键词: rapid testing;    HIV;    lateral flow assay;    upconverting nanoparticles;    diagnostics;    point of care;   

DOI  :  10.3390/s21020330

来源: DOAJ

【 摘 要 】

Rapid diagnostic tests (RDTs) are often used for the detection of anti-human immunodeficiency virus (HIV) antibodies in remote locations in low- and middle-income countries (LMIC) with low or limited access to central laboratories. The typical format of an RDT is a lateral flow assay (LFA) with visual interpretation prone to subjectivity. This risk of misinterpretation can be overcome with luminescent upconverting nanoparticle reporters (UCNPs) measured with a miniaturized easy-to-use reader instrument. An LFA with UCNPs for anti-HIV-1/2 antibodies was developed and the assay performance was evaluated extensively with challenging patient sample panels. Sensitivity (n = 145) of the UCNP-LFA was 96.6% (95% CI: 92.1–98.8%) and specificity (n = 309) was 98.7% (95% CI: 96.7–99.7%). Another set of samples (n = 200) was used for a comparison between the UCNP-LFA and a conventional visual RDT. In this comparison, the sensitivities for HIV-1 were 96.4% (95% CI: 89.8–99.3%) and 97.6% (95% CI: 91.6–99.7%), for the UCNP-LFA and conventional RDT, respectively. The specificity was 100% (95% CI: 96.4–100%) for both assays. The developed UCNP-LFA demonstrates the applicability of UCNPs for the detection of anti-HIV antibodies. The signal measurement is done by a reader instrument, which may facilitate automated result interpretation, archiving and transfer of data from de-centralized locations.

【 授权许可】

Unknown