The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles

Nanomedicine Journal

Seyed Mehdi Kalantar¹  Ehsan Farashahi¹  Mohammad Hassan Sheikhha¹  Ali Jebali²  Negin Saffarzadeh²  Seyed Hossein Hekmatimoghaddam³ 

[1] 1Department of Medical Genetic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;1Department of Medical Genetic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;Department of Laboratory Sciences, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;

关键词: Cervical cancer cells;    DOPE;    Hydroxyapatite nanoparticles;    Oligonucleotide;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective(s): Although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. In this study, hydroxyapatite nanoparticles (NPs) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (DOPE)-modified hydroxyapatite NPs was coated with antisense oligonucleotide of E6 mRNA, and their uptakes into the cervical cancer cell line were evaluated. Materials and Methods: Calcium nitrate and diammonium phosphate were used for the synthesis of the hydroxyapatite nanoparticle. Thus, they were coated with polyethylene glycol (PEG), DOPE and antisense oligonucleotide of E6 mRNA using a cross-linker. Then, hydroxyapatite NPs and DOPE-modified hydroxyapatite NPs were incubated 48 hours with cervical cancer cells and their uptakes were evaluated by fluorescent microscopy. Results: The hydroxyapatite NPs had different shapes and some agglomeration with average size of 100 nm. The results showed DOPE-modified hydroxyapatite NPs had higher uptake than hydroxyapatite NPs (P

【 授权许可】

Unknown