The qSAC3 locus from indica rice effectively increases amylose content under a variety of conditions

BMC Plant Biology

Cailin Wang¹  Lihui Zhou¹  Minghong Gu²  Qianfeng Li²  Changquan Zhang²  Qiaoquan Liu²  Heng Xu³  Liangchao Wang³  Ying Zhu³  Hua Zhang³  Huijie Liu³ 

[1] Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Provincial Key Laboratory of Agrobiology, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences;Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Creative Agriculture, Ministry of Agriculture, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences;

关键词: Rice quality;    Chromosome segment substitution lines;    Amylose content;    Quantitative trait loci;    Environmental effect;   

DOI  :  10.1186/s12870-019-1860-5

来源: DOAJ

【 摘 要 】

Abstract Background Amylose content (AC) is a critical factor for the quality of rice. It is determined by the biosynthesis gene Waxy (Wx) and a variety of quantitative trait loci (QTLs). Although many QTLs have been reported to affect rice AC, few of them have been investigated under varying growth conditions, especially various temperatures, which are known to greatly influence the AC. Results We analyzed the AC at different temperatures and planting seasons in a set of chromosome segment substitution lines (CSSLs) which were derived from a cross between the indica variety 9311 and the japonica variety Nipponbare carrying the same Wx b allele. A joint analysis detected a single locus, qSAC3, with a high logarithm of odds (LOD) score in four different conditions. The qSAC3 from indica 9311 (qSAC3 ind ) substantially increased the AC in japonica Nipponbare under all tested growth conditions. Furthermore, introducing the qSAC3 ind into the soft rice variety Nangeng9108 with Wx mq , a mutant allele of Wx b , also moderately increased its AC and improved its appearance quality significantly by reducing the chalkiness of the polished rice. Conclusions Our results indicate that the qSAC3 ind could increase the AC of japonica rice in different environments as well as in the background of different Wx alleles and that qSAC3 is a valuable locus for fine-tuning the rice AC and ameliorating the dull endosperm in rice varieties with the Wx mq allele.

【 授权许可】

Unknown