Effect of isoamylase application on chemical characteristic of cassava root meal starch

2nd International Conference on Natural Products and Bioresource Sciences - 2018

生物科学;生态环境科学

Puspita, P.S.^1 ; Hermana, W.^1 ; Nahrowi^1

Department Animal Nutrition and Feed Science, Animal Husbandry Faculty, Bogor Agricultural University, Bogor

16680, Indonesia^1

关键词: Amylose content;    Chemical characteristic;    Degree of hydrolysis;    Distilled water;    Enzyme treatment;    High viscosities;    Hydrolyzed products;    Limited information;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/251/1/012058/pdf
DOI  :  10.1088/1755-1315/251/1/012058

来源: IOP

【 摘 要 】

Cassava root meal (CRM) is still rarely used as an ingredient for poultry feed. The high viscosity component can be reduced by enzymatic hydrolysis. However, there is limited information about enzyme treatment on cassava root meal. The objective was to identify and evaluate the effect of isoamylase hydrolysis on the chemical characteristic of hydrolyzed cassava root meal starch which has amylose/amylopectin ratio approached the corn. The cassava root meal was weighed each 200 g containing five levels of isoamylase (CRM-A) 0.01%, (CRM-B) 0.02%, (CRM-C) 0.03%, (CRM-D) 0.04%, (CRM-E) 0.05% (w/w) were moistened with 135 ml distilled water and incubated to at 50 °C for 12 hours. Total starch, amylose, amylopectin, total sugar, and reducing sugar content of hydrolyzed product were measured. The result showed that there were increasing of total starch and amylose content of hydrolyzed cassava root meal and decreasing in amylopectin content and amylose/amylopectin ratio. Reducing sugar increased along with increasing enzyme addition 1.81 - 3.07% DM which corresponds to degree of hydrolysis to 54.52 - 72.99%. It can be concluded that the different levels of isoamylase addition will produce cassava root meal starch with different chemical characteristics. The addition 0.05% isoamylase is able to make amylose/amylopectin ratio of hydrolyzed cassava root meal approached the corn.