Production of the Fragrance Geraniol in Peroxisomes of a Product-Tolerant Baker’s Yeast

Frontiers in Bioengineering and Biotechnology

Dominik Schneider¹  Anja Poehlein¹  Jennifer Gerke²  Maxim Wintergoller²  Gerhard H. Braus²  Holm Frauendorf³  Ziga Zebec⁴  Nigel S. Scrutton⁵  Eriko Takano⁵  Thomas Hofmeister⁶ 

[1] Department of Genomic and Applied Microbiology, Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-Universität Göttingen, Göttingen, Germany;Department of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics, Georg-August-Universität Göttingen, Göttingen, Germany;Institute of Organic and Biomolecular Chemistry, Georg-August-Universität Göttingen, Göttingen, Germany;Molecular Enzymology, Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom;Synthetic Biology Research Centre, SYNBIOCHEM, Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom;Thermo Fisher Scientific GENEART GmbH, Regensburg, Germany;

关键词: geraniol;    peroxisomes;    Saccharomyces cerevisiae;    compartmentalization;    tolerance;    monoterpenoids;   

DOI  :  10.3389/fbioe.2020.582052

来源: DOAJ

【 摘 要 】

Monoterpenoids, such as the plant metabolite geraniol, are of high industrial relevance since they are important fragrance materials for perfumes, cosmetics, and household products. Chemical synthesis or extraction from plant material for industry purposes are complex, environmentally harmful or expensive and depend on seasonal variations. Heterologous microbial production offers a cost-efficient and sustainable alternative but suffers from low metabolic flux of the precursors and toxicity of the monoterpenoid to the cells. In this study, we evaluated two approaches to counteract both issues by compartmentalizing the biosynthetic enzymes for geraniol to the peroxisomes of Saccharomyces cerevisiae as production sites and by improving the geraniol tolerance of the yeast cells. The combination of both approaches led to an 80% increase in the geraniol titers. In the future, the inclusion of product tolerance and peroxisomal compartmentalization into the general chassis engineering toolbox for monoterpenoids or other host-damaging, industrially relevant metabolites may lead to an efficient, low-cost, and eco-friendly microbial production for industrial purposes.

【 授权许可】

Unknown