Biochemical and Immunocytochemical Properties of Peroxisomes and Mitochondria in Bovine Chromaffin Cells

Cell Structure and Function

Naomi Kondo²  Tadao Orii¹  Yasuyuki Suzuki²  Ken Lee³  Nobuyuki Shimozawa² 

[1] Department of Human Welfare, Faculty of Human Welfare, Chubu Gakuin University;Department of Pediatrics, Gifu University School of Medicine;Department of Physiology, Gifu University School of Medicine

关键词: chromaffin cells;    peroxisomes;    mitochondria;    β-oxidation;   

DOI  :  10.1247/csf.22.615

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(21)Cited-By(1)The biochemical and immunocytochemical properties of peroxisomal and mitochondrial β-oxidation enzymes in bovine adrenal chromaffln cells were investigated. Peroxisomes were detectable by immunofluorescence staining using antibodies against acyl-CoA oxidase, peroxisomal 3-ketoacyl-CoA thiolase and catalase. The mitochondria were abundantly stained with antibody against mitochondrial 3-ketoacyl-CoA thiolase. The biosynthesis and intracellular processing of acyl-CoA oxidase and the peroxisomal 3-ketoacyl-CoA thiolase was slower than that in fibroblasts. The peroxisomal β-oxidation activities shown by [1-14C] lignoceric acid oxidation were slightly lower than those in fibroblasts, whereas the mitochondrial β-oxidation activities shown by [1-14C] palmitic acid oxidation were almost identical to those in fibroblasts. Adrenal chromaffin cells are useful materials for investigating the peroxisomal and mitochondrial metabolism of autonomic neurons and may contribute to the clarification of neuronal dysfunction in peroxisomal and mitochondrial disorders.

【 授权许可】

Unknown