Structural Characterization of Flavonoid Glycoconjugates and Their Derivatives with Mass Spectrometric Techniques

Molecules

Łukasz Marczak¹  Maciej Stobiecki¹  Piotr Kachlicki²  Anna Piasecka² 

[1] Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland;Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland;

关键词: collision induced dissociation (CID);    gas chromatography;    flavonoid glycoconjugates;    liquid chromatography;    electrospray ionization—ESI;    matrix assisted laser desorption ionization—MALDI;    mass spectrometry;    soft ionization;    tandem mass spectrometry;   

DOI  :  10.3390/molecules21111494

来源: DOAJ

【 摘 要 】

Mass spectrometry is currently one of the most versatile and sensitive instrumental methods applied to structural characterization of plant secondary metabolite mixtures isolated from biological material including flavonoid glycoconjugates. Resolution of the applied mass spectrometers plays an important role in structural studies of mixtures of the target compounds isolated from biological material. High-resolution analyzers allow obtaining information about elemental composition of the analyzed compounds. Application of various mass spectrometric techniques, including different systems of ionization, analysis of both positive and negative ions of flavonoids, fragmentation of the protonated/deprotonated molecules and in some cases addition of metal ions to the studied compounds before ionization and fragmentation, may improve structural characterization of natural products. In our review we present different strategies allowing structural characterization of positional isomers and isobaric compounds existing in class of flavonoid glycoconjugates and their derivatives, which are synthetized in plants and are important components of the human food and drugs as well as animal feed.

【 授权许可】

Unknown