Design, synthesis and application of fluorogenic probe for detecting l-asparaginase in serum samples

Results in Chemistry

Bo Peng¹  Wei Du²  Bin Fang³  Lin Li⁴  Ding Chen⁴  Naidi Yang⁴  Wenjing Qin⁴  Nanxiang Wang⁴  Qiong Wu⁴  Zhijie Fang⁴  Lan Wang⁴  Congcong Zhang⁴ 

[1] Corresponding authors.;Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, China;;Frontiers Science Center for Flexible Electronics, Xi’an Institute of Flexible Electronics (IFE) and Xi’an Institute of Biomedical Materials &Key Laboratory of Flexible Electronics(KLOFE) ＆ Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (Nanjing Tech), Nanjing 211816, China;

关键词: Fluorogenic probe;    l-asparaginase;    In vitro assay;    Patient serum;   

DOI  :  

来源: DOAJ

【 摘 要 】

Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro l-asparaginase detection. TPAN-Asn exhibited selective and robust response to l-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of l-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of l-asparaginase-based cancer therapy.

【 授权许可】

Unknown