First approach to molecular epidemiology of bovine tuberculosis in Colombia

Revista MVZ Cordoba

Francisco Osorio R.¹  Gloria Puerto-Castro²  Martha Guerrero-Guerrero²  Maira Wintaco M.²  Jimena Jojoa-Jojoa² 

[1] Instituto Colombiano Agropecuario ICA, Programa de Brucelosis y Tuberculosis bovina, Carrera 41 17-81 Bogotá D.C. Colombia.;Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 2651-20 Bogotá D.C. Colombia.;

关键词: Bovine tuberculosis;    Mycobacterium bovis;    diagnostic;    molecular epidemiology;    zoonosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.

【 授权许可】

Unknown