Sustainable Rabbit Skin Glue to Produce Bioactive Nanofibers for Nonactive Wound Dressings

Materials

Ecaterina Matei¹  George Coman¹  Maria Râpă¹  AndraMihaela Predescu¹  Cristian Predescu¹  AndreiConstantin Berbecaru¹  Simona Savin²  Roxana Constantinescu³  Carmen Gaidau³  MarianaDaniela Berechet³ 

[1] Faculty of Materials Science and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania;National Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, Sector 6, 060031 Bucharest, Romania;National Research and Development Institute for Textiles and Leather-Division Leather and Footwear Research Institute, 031215 Bucharest, Romania;

关键词: collagen rabbit glue;    antimicrobial agents;    electrospinning;    biocompatibility;    antimicrobial activity;    antioxidant activity;   

DOI  :  10.3390/ma13235388

来源: DOAJ

【 摘 要 】

This paper assessed the collagen glue (Col) from rabbit skin for use as a raw material in combination with different water-based dispersants of antimicrobial agents such as ZnO NPs, TiO₂ NPs doped with nitrogen and Ag NPs (TiO₂-N-Ag NPs), and chitosan (CS) for the production of biocompatible and antimicrobial nanofibers. The electrospun nanofibers were investigated by scanning electron microscopy (SEM), attenuated total reflectance in conjunction with Fourier-transform infrared spectroscopy (ATR-FT-IR) analyses and antioxidant activity. The biocompatibility of electrospun nanofibers was investigated on cell lines of mouse fibroblast NCTC ((clone L929) using MTT test assays. Antimicrobial activity was performed against Escherichia coli and Staphylococcus aureus bacteria and Candida albicans pathogenic fungus. Electrospun antimicrobial nanofibers based on collagen glue achieved reduction in the number of viable microorganisms against both fungi and bacteria and exhibited multiple inhibitory actions of fungal and bacterial strains. The electrospun nanofibers showed average dimension sizes in the range of 30–160 nm. The results indicated that both Col/TiO₂-N-Ag NPs and Col/CS formulations are suitable for cell proliferation and may be useful for producing of nonactive wound dressings.

【 授权许可】

Unknown