iTRAQ-Based Quantitative Proteomics Analysis on Rice Anther Responding to High Temperature

International Journal of Molecular Sciences

Haoliang Yan¹  Wenying Zhang¹  Yunbo Zhang¹  Ke Liu¹  Xiaohai Tian¹  Qilin Mu¹  Tsutomu Matsui²  Pingfang Yang³ 

[1] Agricultural College, Yangtze University, Jingzhou 434025, China;Applied Biological Faculty, Gifu University, Gifu 501-1193, Japan;Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;

关键词: rice;    high temperature;    iTRAQ;    proteomics;    spikelet fertility;   

DOI  :  10.3390/ijms18091811

来源: DOAJ

【 摘 要 】

As one of the most important crops, rice provides the major food for more than half of the world population. However, its production is limited by many environmental factors, among which high temperature stress (HS) frequently occurs during anthesis and reduces its spikelet fertility. To explore the mechanism of HS tolerance in rice, we conducted a comparative proteomics analysis on the anthers between HS resistant and sensitive cultivars under different levels of high temperature. Under the same HS treatment, the resistant cultivar showed much higher spikelet fertility than the sensitive cultivar. Proteomic data showed that HS lead to the degradation of ribosomal proteins in the sensitive cultivar but not in the resistant one, which might result in the injury of protein biosynthetic machinery. In contrast, HS induced the increase of sHSP, β-expansins and lipid transfer proteins in the resistant cultivar, which might contribute to its ability to tolerate HS. The results provide some new insights into the mechanism of rice HS response.

【 授权许可】

Unknown