Characterization of Scenedesmus obtusiusculus AT-UAM for high-energy molecules accumulation: deeper insight into biotechnological potential of strains of the same species

Biotechnology Reports

Marcia Morales¹  Alma Toledo-Cervantes²  Gloria Garduño Solórzano³  Jorge E. Campos⁴  Martha Martínez-García⁴ 

[1]Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Ciudad de México, Mexico

[2]Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana – Iztapalapa, Ciudad de México, Mexico

[3]Herbario IZTA, FES Iztacala, UNAM, Mexico

[4]Lab. Bioquímica Molecular, UBIPRO, FES Iztacala, UNAM, Mexico

关键词: Carbohydrates;    ITS2;    Lipids;    Morphological characterization;    Scenedesmus obtusiusculus;   

DOI  :  10.1016/j.btre.2017.11.009

来源: DOAJ

【 摘 要 】

Scenedesmus obtusiusculus AT-UAM, isolated from Cuatro Ciénegas wetlands in Mexico was taxonomically, molecularly and biochemically compared to S. obtusiusculus CCAP 276/25 (Culture Collection of Algae and Protozoa, Scotland, UK). Analysis of Internal Transcribed Spacer 2 (ITS2) secondary structures confirmed that the mexican strain belongs to S. obtusiusculus with one change in the ITS2 nucleotide sequence. However, both strains exhibited different biochemical and fatty acid profiles and therefore biotechnological potential, emphasizing the need for deeper studies among strains of the same species. Furthermore, the biochemical variations of S. obtusiusculus AT-UAM under nitrogen starvation and different levels of irradiance were evaluated. The maximum lipid production (1730 mg L−1) was obtained at 613 μmol m−2 s−1 while the highest carbohydrate content (49%) was achieved at 896 μmol m−2 s−1. Additionally, this strain was capable of storing lipids (∼52%) and carbohydrates (∼40%) under outdoor condition depending on the light availability in the cultivation broth.

【 授权许可】

Unknown