Cytochrome P450 and Glutathione S-Transferase Confer Metabolic Resistance to SYP-14288 and Multi-Drug Resistance in Rhizoctonia solani

Frontiers in Microbiology

Tongshan Cui¹  Tan Dai¹  Weizhen Wang¹  Pengfei Liu¹  Xili Liu¹  Xingkai Cheng¹  Zhihong Hu²  Ping Han²  Jianjun Hao³  Maolin Hu⁴ 

[1] Department of Plant Pathology, China Agricultural University, Beijing, China;Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China;School of Food and Agriculture, University of Maine, Orono, ME, United States;Shenzhen Agricultural Technology Promotion Center, Shenzhen, China;

关键词: uncoupler;    metabolic resistance;    multi-drug resistance;    Rhizoctonia solani;    cytochrome P450s;    glutathione-S-transferas;   

DOI  :  10.3389/fmicb.2022.806339

来源: DOAJ

【 摘 要 】

SYP-14288 is a fungicide as an uncoupler of oxidative phosphorylation, which is effective in controlling fungal pathogens like Rhizoctonia solani. To determine whether R. solani can develop SYP-14288 resistance and possibly multi-drug resistance (MDR), an SYP-14288-resistant mutant of R. solani X19-7 was generated from wild-type strain X19, and the mechanism of resistance was studied through metabolic and genetic assays. From metabolites of R. solani treated with SYP-14288, three compounds including M1, M2, and M3 were identified according to UPLC-MS/MS analysis, and M1 accumulated faster than M2 and M3 in X19-7. When X19-7 was treated by glutathione-S-transferase (GST) inhibitor diethyl maleate (DEM) and SYP-14288 together, or by DEM plus one of tested fungicides that have different modes of action, a synergistic activity of resistance occurred, implying that GSTs promoted metabolic resistance against SYP-14288 and therefore led to MDR. By comparing RNA sequences between X19-7 and X19, six cytochrome P450s (P450s) and two GST genes were selected as a target, which showed a higher expression in X19-7 than X19 both before and after the exposure to SYP-14288. Furthermore, heterologous expression of P450 and GST genes in yeast was conducted to confirm genes involved in metabolic resistance. In results, the P450 gene AG1IA_05136 and GST gene AG1IA_07383 were related to fungal resistance to multiple fungicides including SYP-14288, fluazinam, chlorothalonil, and difenoconazole. It was the first report that metabolic resistance of R. solani to uncouplers was associated with P450 and GST genes.

【 授权许可】

Unknown