Utilizing Bacillus to inhibit the growth and infection by sheath blight pathogen, Rhizoctoniasolani in rice

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Margani, R.^1 ; Hadiwiyono^1 ; Widadi, S.^1

Agrotechnology Department, Agricultural Faculty, Sebelas Maret University Surakarta, Indonesia^1

关键词: Biological control agent;    Control measures;    Control methods;    Potato-dextrose;    R. Solani;    Rhizoctonia solani;    Rice fields;    Sheath blight;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012070/pdf
DOI  :  10.1088/1755-1315/142/1/012070

来源: IOP

【 摘 要 】

Rhizoctonia solani Kuhn is a common pathogen of rice. The pathogen causes sheath blight of rice. The pathogen can cause loss in the production of rice up to 45%. So far, the disease however is still poorly taken care of by the farmers and researchers, so the control measures is nearly never practiced by the farmers in the fields. It due to the unavailability of effective control method of the disease. Therefore, development to control the disease is important. Bacillus is one of popular bacteria which is effective as biological control agent of a lot of pathogens in plants, but it has not been used for control sheath blight in rice yet. The current researches were aimed to study the potential of Bacillus collected from healthy rice as candidates of biological control agent of the disease. The results showed that some isolates showed indications to inhibit significantly the growth and infection of the pathogen. We obtained at least five isolates of Bacillus collected from leaves, sheath, and stem of healthy rice fields. All of the isolates could effectively inhibit the growth of R. solani in vitro on potato dextrose medium at range 30.33-58.00%, whereas in vivo B05 isolate was the most effective in inhibiting the infection of pathogen at 30.43%. It was not significantly different (P≥0.05) to application of hexaconazol with dosage of 2 ml L-1.