Glutathione–Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair

International Journal of Molecular Sciences

Silvia Buonvino¹  Sonia Melino¹  Emilia Di Giovanni¹  Ivano Amelio² 

[1] Department of Chemical Science and Technologies, University of Rome Tor Vergata, via della Ricerca Scientifica 1, 00133 Rome, Italy;MRC Toxicology Unit, University of Cambridge, Cambridge CB2 1QP, UK;

关键词: hydrogen sulfide;    garlic;    regenerative medicine;    oxidative stress;    mscs;    organosulfur compounds;    cell migration;    cell differentiation;   

DOI  :  10.3390/ijms21051638

来源: DOAJ

【 摘 要 】

The endogenous gasotransmitter H₂S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H₂S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H₂S-releasing agents on the growth of stem cells have not been fully investigated. H₂S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H₂S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H₂S agents on the cell growth and differentiation of cardiac Lin⁻ Sca1⁺ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH−garlic conjugates (GSGa) on cMSC compared to other H₂S-releasing agents, such as Na₂S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell−cell adhesion and commitment to differentiation. These results highlight the effects of H₂S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.

【 授权许可】

Unknown