Immobilization of Phospholipase D on Silica-Coated Magnetic Nanoparticles for the Synthesis of Functional Phosphatidylserine

Catalysts

Xiangzhao Mao¹  Haiyang Zhang¹  Changhu Xue¹  Wen-can Huang¹  Zhen Liu¹  Qingqing Han¹  Jianan Sun¹ 

[1] College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;

关键词: immobilization;    phospholipase D;    magnetic nanoparticles;    stability;    phosphatidylserine;   

DOI  :  10.3390/catal9040361

来源: DOAJ

【 摘 要 】

In this study, silica-coated magnetic nanoparticles (Fe₃O₄/SiO₂) were synthesized and applied in the immobilization of phospholipase D (PLD_a2) via physical adsorption and covalent attachment. The immobilized PLD_a2 was applied in the synthesis of functional phosphatidylserine (PS) through a transphophatidylation reaction. The synthesis process and characterizations of the carriers were examined by scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier-transform infrared spectroscopy (FT-IR). The optimum immobilization conditions were evaluated, and the thermal and pH stability of immobilized and free PLD_a2 were measured and compared. The tolerance to high temperature of immobilized PLD_a2 increased remarkably by 10°C. Furthermore, the catalytic activity of the immobilized PLD_a2 remained at 40% after eight recycles, which revealed that silica-coated magnetic nanoparticles have potential application for immobilization and catalytic reactions in a biphasic system.

【 授权许可】

Unknown