Synthesis and Application of Amine Functionalized Iron Oxide Nanoparticles on Menaquinone-7 Fermentation: A Step towards Process Intensification

Nanomaterials

Alireza Ebrahiminezhad²  Vikas Varma¹  Shuyi Yang¹  Younes Ghasemi³  Aydin Berenjian¹ 

[1] School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand;Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa 74615, Iran;Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71348, Iran;

关键词: immobilization;    downstream processing;    magnetic nanoparticles;    MK-7;    bioseparations;    fermentation;   

DOI  :  10.3390/nano6010001

来源: mdpi

【 摘 要 】

Industrial production of menaquione-7 by Bacillus subtilis natto is associated with major drawbacks. To address the current challenges in menaquione-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified menqainone-7 process. This article introduces the new concept of production and application of l-lysine coated iron oxide nanoparticles (l-Lys@IONs) as a novel tool for menaquinone-7 biosynthesis. l-Lys@IONs with the average size of 7 nm were successfully fabricated and were examined in a fermentation process of l-Lys@IONs decorated Bacillus subtilis natto. Based on the results, higher menaquinone-7 specific yield was observed for l-Lys@IONs decorated bacterial cells as compared to untreated bacteria. In addition, more than 92% removal efficacy was achieved by using integrated magnetic separation process. The present study demonstrates that l-Lys@IONs can be successfully applied during a fermentation of menaquinone-7 without any negative consequences on the culture conditions. This study provides a novel biotechnological application for IONs and their future role in bioprocess intensification.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.