Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry

BMC Plant Biology

Timothy Johnson¹  Michael Schwieterman¹  Thomas A. Colquhoun¹  Jinhe Bai²  Anne Plotto²  Jeremy Pillet³  Christopher Barbey³  Alan H. Chambers³  Kevin M. Folta³  Zhilong Bao³  Benjamin Harrison³  Vance M. Whitaker³ 

[1] Environmental Horticultural Sciences Department, University of Florida;Horticultural Research Laboratory, Agriculture Research Service, USDA;Horticultural Sciences Department, University of Florida;

关键词: Aroma;    Flavor;    Fragaria;    Grape;    Methyl anthranilate;    Molecular maker;   

DOI  :  10.1186/s12870-017-1088-1

来源: DOAJ

【 摘 要 】

Abstract Background Methyl anthranilate (MA) contributes an attractive fruity note to the complex flavor and aroma of strawberry (Fragaria spp.), yet it is rare in modern cultivars. The genetic basis for its biosynthesis has not been elucidated. Understanding the specific genes required for its synthesis could allow  the development of gene/allele-specific molecular markers to speed breeding of flavorful strawberries. Results Ripe fruits from individuals in an F1 population resulting from a cross between a MA producer and a non-producer were examined using a bulk-segregant transcriptome approach. MA producer and non-producer transcriptomes were compared, revealing five candidate transcripts that strictly co-segregated with MA production. One candidate encodes an annotated methyltransferase. MA levels are lower when this transcript is suppressed with RNAi, and bacterial cultures expressing the protein produced MA in the presence of anthranilic acid. Frozen fruit powders reconstituted with anthranilic acid and a methyl donor produced MA only if the transcript was detected in the fruit powder. A DNA-based molecular marker was developed that segregates with the MA-producing gene variant. Conclusions These analyses indicate that the methyltransferase, now noted ANTHRANILIC ACID METHYL TRANSFERASE (FanAAMT), mediates the ultimate step of MA production in cultivated strawberry. Identification of this gene and its associated molecular marker may hasten breeding efforts to introduce this important volatile into modern cultivars.

【 授权许可】

Unknown