Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes

Journal of Genetic Engineering and Biotechnology

Mona A. Esawy¹  Nahla M. Mansour¹  Abdelhamid A. Hamdy¹  Nouran A. Elattal¹  Ghada E. A. Awad¹  Magdy A. Amin²  Amal E. Ali²  Ian Connerton³ 

[1] Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre;Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University;Division of Food Sciences, School of Biosciences, University of Nottingham Loughborough;

关键词: Genome sequence;    Bacillus subtilis;    Probiotics;    Honey;    Honeybee;   

DOI  :  10.1186/s43141-020-00050-w

来源: DOAJ

【 摘 要 】

Abstract Background We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains. Results The complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics. Conclusions The comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution. Significance and impact of study We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications

【 授权许可】

Unknown