Identification of Radiation-Induced miRNA Biomarkers Using the CGL1 Cell Model System

Bioengineering

Christopher D. McTiernan¹  Simon J. Lees²  Neelam Khaper²  Jayden Peterson³  Tze Chun Tai³  Sujeenthar Tharmalingam³  Douglas R. Boreham³  Christopher Thome³ 

[1] Medical Sciences Division, NOSM University, 935 Ramsey Lake Rd., Sudbury, ON P3E 2C6, Canada;Medical Sciences Division, NOSM University, 955 Oliver Rd., Thunder Bay, ON P7B 5E1, Canada;School of Natural Sciences, Laurentian University, Sudbury, ON P3E 2C6, Canada;

关键词: miRNA;    epigenetics;    low-dose radiation;    next-generation sequencing;    miRNAome;    CGL1;   

DOI  :  10.3390/bioengineering9050214

来源: DOAJ

【 摘 要 】

MicroRNAs (miRNAs) have emerged as a potential class of biomolecules for diagnostic biomarker applications. miRNAs are small non-coding RNA molecules, produced and released by cells in response to various stimuli, that demonstrate remarkable stability in a wide range of biological fluids, in extreme pH fluctuations, and after multiple freeze–thaw cycles. Given these advantages, identification of miRNA-based biomarkers for radiation exposures can contribute to the development of reliable biological dosimetry methods, especially for low-dose radiation (LDR) exposures. In this study, an miRNAome next-generation sequencing (NGS) approach was utilized to identify novel radiation-induced miRNA gene changes within the CGL1 human cell line. Here, irradiations of 10, 100, and 1000 mGy were performed and the samples were collected 1, 6, and 24 h post-irradiation. Corroboration of the miRNAome results with RT-qPCR verification confirmed the identification of numerous radiation-induced miRNA expression changes at all doses assessed. Further evaluation of select radiation-induced miRNAs, including miR-1228-3p and miR-758-5p, as well as their downstream mRNA targets, Ube2d2, Ppp2r2d, and Id2, demonstrated significantly dysregulated reciprocal expression patterns. Further evaluation is needed to determine whether the candidate miRNA biomarkers identified in this study can serve as suitable targets for radiation biodosimetry applications.

【 授权许可】

Unknown