Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen

Sensors

Niko Hildebrandt¹  Olivier Lefebvre¹  Karl David Wegner¹  Shashi Bhuckory¹  Xue Qiu¹ 

[1] NanoBioPhotonics, Institut d’Electronique Fondamentale, Université Paris-Saclay, Université Paris-Sud, CNRS, 91405 Orsay, France;

关键词: Qdots;    antibodies;    bioconjugation;    terbium;    FRET;    time-gating;    homogeneous;    immunoassay;    serum;    TPSA;    clinical diagnostics;   

DOI  :  10.3390/s16020197

来源: DOAJ

【 摘 要 】

The integration of semiconductor quantum dots (QDs) into homogeneous Förster resonance energy transfer (FRET) immunoassay kits for clinical diagnostics can provide significant advantages concerning multiplexing and sensitivity. Here we present a facile and functional QD-antibody conjugation method using three commercially available QDs with different photoluminescence (PL) maxima (605 nm, 655 nm, and 705 nm). The QD-antibody conjugates were successfully applied for FRET immunoassays against prostate specific antigen (PSA) in 50 µL serum samples using Lumi4-Tb (Tb) antibody conjugates as FRET donors and time-gated PL detection on a KRYPTOR clinical plate reader. Förster distance and Tb donor background PL were directly related to the analytical sensitivity for PSA, ...which resulted in the lowest limits of detection for Tb-QD705 (2 ng/mL), followed by Tb-QD655 (4 ng/mL), and Tb-QD605 (23 ng/mL). Duplexed PSA detection using the Tb-QD655 and Tb-QD705 FRET-pairs demonstrated the multiplexing ability of our immunoassays. Our results show that FRET based on QD acceptors is suitable for multiplexed and sensitive biomarker detection in clinical diagnostics.

【 授权许可】

Unknown