miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells

Frontiers in Genetics

Jin Huang¹  Caixia Peng¹  Yu Ding¹  Xiaoyan Shi¹  Jinrong He¹  Xiaolu Mao²  Zihui Xu³  Lan Xiao⁴ 

[1] Central Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;Department of Clinical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;;Department of Endocrinology &Department of Obstetrics and Gynecology, The First Affiliated Hospital, An Hui Medical University, Hefei, China;Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;

关键词: miR-205-5p;    PTEN;    cisplatin resistance;    ovarian cancer;    AKT;   

DOI  :  10.3389/fgene.2018.00555

来源: DOAJ

【 摘 要 】

Cisplatin resistance is a major cause of treatment failure in advanced ovarian cancer. The limited evidence shows the paradoxical regulation of miR-205 on chemotherapy resistance in cancer. Herein, we found that miR-205-5p was enormously increased in cisplatin-resistant C13K ovarian cancer cells compared with its cisplatin-sensitive OV2008 parental cells using miRNA microarrays, which was further verified by quantitative PCR. Furthermore, we confirmed that inhibition of miR-205-5p upregulated PTEN and subsequently attenuated its downstream target p-AKT, which inversed C13K cells from cisplatin resistance to sensitivity. Our data suggest that miR-205-5p contributes to cisplatin resistance in C13K ovarian cancer cells may via targeting PTEN/AKT pathway.

【 授权许可】

Unknown