Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation

Molecules

Ruth Medeiros Oliveira¹  Rafael Barros Gomes Camara¹  Mariana Santana Santos Pereira Costa¹  Jessika Fernanda Santiago Monte¹  Júlia Moraes Fernandes²  Daniele de Souza Marinho do Nascimento³  Dayanne Lopes Gomes³  Hugo Alexandre Oliveira Rocha³  Silvana Maria Zucolotto Langassner³ 

[1] Departamento de Bioquimica, Centro de Biociencias, Universidade Federal do Rio Grande do Norte-UFRN, Av. Salgado Filho 3000, Natal-RN 59078-970, Brazil;Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte-UFRN, Rua General Gustavo Cordeiro de Farias, s/n, Natal-RN 59012-570, Brazil;Programa de Pós-graduação em Ciencias da Saúde, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte-UFRN, R. Gen. Gustavo Cordeiro de Farias, s/n, Natal-RN 59012-570, Brazil;

关键词: adipogenesis;    antioxidant;    chlorogenic acid;   

DOI  :  10.3390/molecules22060972

来源: DOAJ

【 摘 要 】

Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that B. trimera has an anti-adipogenic effect and could be used in the development of functional foods.

【 授权许可】

Unknown