Illumina and PacBio DNA sequencing data, de novo assembly and annotation of the genome of Aurantiochytrium limacinum strain CCAP_4062/1

Data in Brief

Alberto Amato¹  Christian Morabito²  Fabrice Rébeillé²  Eric Maréchal²  Riccardo Aiese Cigliano³ 

[1] Corresponding authors.;Laboratoire de Physiologie Cellulaire Végétale, Université Grenoble Alpes, CEA, CNRS, INRAE, IRIG-LPCV, 38054 Grenoble Cedex 9, France;Sequentia Biotech Carrer d'Àlaba, 61, 08005 Barcelona, Spain;

关键词: Genome;    Third generation sequencing;    Next generation sequencing;    Structural annotation;    Biotechnology;    Thraustochytrid;   

DOI  :  

来源: DOAJ

【 摘 要 】

The complete genome of the thraustochytrid Aurantiochytrium limacinum strain CCAP_4062/1 was sequenced using both Illumina Novaseq 6000 and third generation sequencing technology PacBio RSII in order to obtain trustworthy assembly and annotation. The reads from both platforms were combined at multiple levels in order to obtain a reliable assembly, then compared to the A. limacinum ATCCⓇ MYA1381™ reference genome. The final assembly was annotated with the help of strain CCAP_4062/1 RNAseq data. A. limacinum strain CCAP_4062/1 is an industrial strain used for the production of very long chain polyunsaturated fatty acids, like the docosahexaenoic acid that is an essential fatty acid synthesised only at very low pace in humans and vertebrates . Thraustochytrids in general and Aurantiochytrium more specifically, are used for carotenoid and squalene production as well. Beside their biotechnological interest, thraustochytrids play a crucial role in both inshore and oceanic basins ecosystems. Genome sequences will foster biotechnological as well as ecological studies.

【 授权许可】

Unknown