Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Journal of Functional Foods

Martine Schroyen¹  Nadia Everaert²  Julie Uerlings²  Els Willems²  Sofie Tanghe³  Geert Bruggeman³  Jérôme Bindelle³ 

[1] Research Foundation for Industry and Agriculture, National Scientific Research Foundation (FRIA-FNRS), Brussels, Belgium;Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, Gembloux, Belgium;Royal Agrifirm Group, Apeldoorn, the Netherlands;

关键词: Prebiotic;    In vitro fermentation;    Porcine intestinal epithelial cells;    Short-chain fatty acids;    Gut barrier;    Inflammatory response;   

DOI  :  

来源: DOAJ

【 摘 要 】

Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.

【 授权许可】

Unknown