Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

eLife

Elizabeth A Warren¹  Niles P Donegan²  Kaitlin D Yarrington²  Dominique H Limoli²  Ambrose L Cheung³  George A O'Toole³ 

[1] Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, United States;Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, United States;Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, United States;

关键词: Pseudomonas aeruginosa;    Staphylococcus aureus;    polymicrobial infections;    bacterial motility;    cystic fibrosis;   

DOI  :  10.7554/eLife.47365

来源: DOAJ

【 摘 要 】

Microbes often live in multispecies communities where interactions among community members impact both the individual constituents and the surrounding environment. Here, we developed a system to visualize interspecies behaviors at initial encounters. By imaging two prevalent pathogens known to be coisolated from chronic illnesses, Pseudomonas aeruginosa and Staphylococcus aureus, we observed P. aeruginosa can modify surface motility in response to secreted factors from S. aureus. Upon sensing S. aureus, P. aeruginosa transitioned from collective to single-cell motility with an associated increase in speed and directedness – a behavior we refer to as ‘exploratory motility’. Explorer cells moved preferentially towards S. aureus and invaded S. aureus colonies through the action of the type IV pili. These studies reveal previously undescribed motility behaviors and lend insight into how P. aeruginosa senses and responds to other species. Identifying strategies to harness these interactions may open avenues for new antimicrobial strategies.

【 授权许可】

Unknown