Frontiers in Neuroscience | |
Optically transparent multi–suction electrode arrays | |
John Michael Nagarah1  Hiroki eAsari1  Daniel A. Wagenaar1  Rell eParker1  Annette eStowasser2  | |
[1] California Institute of Technology;University of Cincinnati; | |
关键词: Electrophysiology; Retina; multielectrode array; leech; neural interface; brain slice; | |
DOI : 10.3389/fnins.2015.00384 | |
来源: DOAJ |
【 摘 要 】
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to the tissue; although, such arrays are not optically transparent, limiting their potential to be combined with optical-based technologies. We present here multi-suction electrode arrays (MSEAs) in quartz that yield a substantial increase in the detected number of units and unit signal to noise ratio from mouse cortico-hippocampal slices and mouse retina explants. This enables the visualization of stronger cross correlations between the firing rates of the various sources. Additionally, the MSEA's transparency allows us to record voltage sensitive dye activity from a leech ganglion with single neuron resolution using widefield microscopy simultaneously with the electrode array recordings. The combination of enhanced electrical signals and compatibility with optical-based technologies should make the MSEA a valuable tool for investigating neuronal circuits.
【 授权许可】
Unknown