Complementarity of Matrix- and Nanostructure-Assisted Laser Desorption/Ionization Approaches

Nanomaterials

Pawel Pomastowski¹  Boguslaw Buszewski¹ 

[1] Interdisciplinary Center for Modern Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100 Torun, Poland;

关键词: matrix-assisted laser desorption/ionization;    nanostructures-assisted laser desorption/ionization;    MALDI;    NALDI;    bioanalytics;    nanoparticles;   

DOI  :  10.3390/nano9020260

来源: DOAJ

【 摘 要 】

In recent years, matrix-assisted laser desorption/ionization (MALDI) has become the main tool for the study of biological macromolecules, such as protein nano-machines, especially in the determination of their molecular masses, structure, and post-translational modifications. A key role in the classical process of desorption and ionization of the sample is played by a matrix, usually a low-molecular weight weak organic acid. Unfortunately, the interpretation of mass spectra in the mass range of below m/z 500 is difficult, and hence the analysis of low molecular weight compounds in a matrix-assisted system is an analytical challenge. Replacing the classical matrix with nanomaterials, e.g., silver nanoparticles, allows improvement of the selectivity and sensitivity of spectrometric measurement of biologically important small molecules. Nowadays, the nanostructure-assisted laser desorption/ionization (NALDI) approach complements the classic MALDI in the field of modern bioanalytics. In particular, the aim of this work is to review the recent advances in MALDI and NALDI approaches.

【 授权许可】

Unknown