A novel autoantibody targeting calreticulin is associated with cancer in patients with idiopathic inflammatory myopathies

Clinical & Translational Immunology

Qiu‐Xiang Cheng¹  Heng Yang¹  Gen‐Hong Cheng²  Qing‐Lin Peng³  Yong‐Peng Ge³  Ya‐Mei Zhang³  Guo‐Chun Wang³  He Chen³  Xin Lu³ 

[1] Center for Systems Medicine Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China;Department of Microbiology, Immunology and Molecular Genetics University of California Los Angeles CA 90095 USA;Department of Rheumatology China‐Japan Friendship Hospital Beijing 100029 China;

关键词: anti‐calreticulin autoantibodies;    biomarkers;    idiopathic inflammatory myopathy;    malignancy;   

DOI  :  10.1002/cti2.1195

来源: DOAJ

【 摘 要 】

Abstract Objectives To investigate the prevalence and clinical significance of anti‐calreticulin autoantibodies (anti‐CRT Ab) in a large cohort of idiopathic inflammatory myopathy (IIM) patients. Methods Sera from 469 patients with IIM, 196 patients with other connective tissue diseases, 28 patients with solid tumors and 81 healthy controls were screened for anti‐CRT Ab by enzyme‐linked immunosorbent assay using human recombinant CRT protein. Sera from 35 IIM patients were tested using an immunoprecipitation assay to confirm the presence of anti‐CRT Ab. Subsequently, IIM–cancer patients were identified and divided into new‐onset, remission and recurrent groups based on their cancer status. The relationships between anti‐CRT Ab levels and IIM disease activity were also investigated. Results Serum anti‐CRT Ab was detected positive in 81 of the 469 (17.3%) IIM patients. Immunoprecipitated bands were observed at a molecular weight of 60 kDa corresponding to the CRT protein. The IIM patients with anti‐CRT Ab more frequently had cancers compared to the patients without anti‐CRT Ab. Moreover, the prevalence of anti‐CRT Ab differed according to the cancer status. The IIM patients with recurrent cancers had a much higher prevalence of anti‐CRT Ab than those with cancers in remission. Also, serum anti‐CRT Ab levels positively correlated with disease activity at baseline and at follow‐up visits. Conclusion We report the existence of serum anti‐CRT Ab in IIM patients and demonstrate the possible association of anti‐CRT Ab with malignancy in IIM patients. Serum anti‐CRT Ab could serve as a novel candidate marker of cancer in IIM patients.

【 授权许可】

Unknown