Alteration of Tight Junction Gene Expression by Calcium-and Vitamin D-Deficient Diet in the Duodenum ofCalbindin-Null Mice

International Journal of Molecular Sciences

Beum-Soo An¹  Eui-Ju Hong²  Eui-Bae Jeung²  Inho Hwang²  Hong-Seok Kang²  Hyun Yang²  Changhwan Ahn² 

[1] Department of Biomaterials Science, College of National Resources & Life Science,Pusan National University, 1268-50 Samrangjin-ro, Samrangjin-eup, Miryang-si,Gyeongsangnam-do 627-706, Korea;Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea;

关键词: calbindin;    transcellular pathway;    paracellular pathway;    calcium absorption;    tight junction;   

DOI  :  10.3390/ijms141122997

来源: DOAJ

【 摘 要 】

Calcium absorption is regulated by both active (transcellular) and passive (paracellular) pathways. Although each pathway has been studied, correlations between the two pathways have not been well elucidated. In previous investigations, the critical transcellular proteins, calbindin-D9k (CaBP-9k) and -D28k (CaBP-28k), were shown to affect other transcellular pathways by buffering intracellular calcium concentrations. The rate of paracellular calcium transport in the duodenum is generally determined by the expression of tight junction genes. In the present study, the effect of dietary calcium and/or vitamin D supplementation on the expression of tight junction genes (occludin, ZO-1 and claudin 2, 10b, 12 and 15) in the duodenum of CaBP-9k- and/or -28k-deficient mice was examined. With a normal diet, the expression of most tight junction genes in the duodenum was significantly increased in CaBP-9k knockout (KO) mice compared to wild-type (WT) animals. With a calcium- and vitamin D-deficient diet, tight junction gene expression was significantly decreased in the duodenum of the CaBP-9k KO mice. These findings suggest that expression of paracellular tight junction genes is regulated by transcellular CaBP proteins, suggesting that active and passive calcium transport pathways may function cooperatively.

【 授权许可】

Unknown