Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice

International Journal of Molecular Sciences

Inho Hwang¹  Hyun Yang¹  Hong-Seok Kang¹  Changhwan Ahn¹  Eui-Ju Hong¹  Beum-Soo An² 

[1] Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea; E-Mails:;Department of Biomaterials Science, College of National Resources & Life Science, Pusan National University, 1268-50 Samrangjin-ro, Samrangjin-eup, Miryang-si, Gyeongsangnam-do 627-706, Korea; E-Mail:

关键词: calbindin;    transcellular pathway;    paracellular pathway;    calcium absorption;    tight junction;   

DOI  :  10.3390/ijms141122997

来源: mdpi

【 摘 要 】

Calcium absorption is regulated by both active (transcellular) and passive (paracellular) pathways. Although each pathway has been studied, correlations between the two pathways have not been well elucidated. In previous investigations, the critical transcellular proteins, calbindin-D_9k (CaBP-9k) and -D_28k (CaBP-28k), were shown to affect other transcellular pathways by buffering intracellular calcium concentrations. The rate of paracellular calcium transport in the duodenum is generally determined by the expression of tight junction genes. In the present study, the effect of dietary calcium and/or vitamin D supplementation on the expression of tight junction genes (occludin, ZO-1 and claudin 2, 10b, 12 and 15) in the duodenum of CaBP-9k- and/or -28k-deficient mice was examined. With a normal diet, the expression of most tight junction genes in the duodenum was significantly increased in CaBP-9k knockout (KO) mice compared to wild-type (WT) animals. With a calcium- and vitamin D-deficient diet, tight junction gene expression was significantly decreased in the duodenum of the CaBP-9k KO mice. These findings suggest that expression of paracellular tight junction genes is regulated by transcellular CaBP proteins, suggesting that active and passive calcium transport pathways may function cooperatively.

【 授权许可】

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