Protective effect of metformin on testicular ischemia/reperfusion injury in rats

Acta Cirúrgica Brasileira

关键词: Testis;    Ischemia;    Reperfusion;    Metformin;    Rats.;   

DOI  :  10.1590/S0102-865020160060000008

来源: DOAJ

【 摘 要 】

ABSTRACTPURPOSE: To investigate the protective effect of metformin on testicular ischemia/reperfusion (I/R) injury in rats.METHODS: Eighteen adult male Wistar rats were randomly divided into three experimental groups (n=6), as follows: Sham, I/R, and Metformin. 1-hour ischemia was induced by the left testicular artery and vein clipping followed by 7 days of reperfusion. Metformin (100 mg/kg) was administrated orally for 7 days via oral gavage after ischemic period. At the end of trial, the left testis was removed for histological analysis and oxidative stress measurement.RESULTS: I/R reduced superoxide dismutase (SOD) activities and testicular Johnsen's scores accompanied by an elevation in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in comparison with the sham group (P < 0.05). Compared to I/R group, metformin restored testicular Johnsen's scores, SOD activity, MDA and MPO levels (P < 0.05).CONCLUSION: Metformin has a protective effect against I/R injury on the testis.

【 授权许可】

Unknown